{"id":23592,"date":"2024-05-21T14:27:08","date_gmt":"2024-05-21T12:27:08","guid":{"rendered":"https:\/\/www.carnisostenibili.it\/?p=23592"},"modified":"2024-05-21T14:27:08","modified_gmt":"2024-05-21T12:27:08","slug":"the-importance-of-polyphenols-and-tannins-in-meat","status":"publish","type":"post","link":"https:\/\/www.carnisostenibili.it\/en\/the-importance-of-polyphenols-and-tannins-in-meat\/","title":{"rendered":"The importance of polyphenols (and tannins) in meat"},"content":{"rendered":"<p align=\"JUSTIFY\"><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><i>Polyphenols are natural antioxidants with beneficial effects on human health and have recently become highly sought after by consumers in food products.<\/i><\/span><\/span><\/span><\/p>\n<p align=\"JUSTIFY\"><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">Polyphenols are <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>natural antioxidants<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> with beneficial effects on <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>human health<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and have recently become highly sought after by consumers in food products. The richest foods in <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>polyphenols<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> are fruits and vegetables, as these plant compounds are formed by plant metabolism and play various roles in growth, colour formation, reproduction and <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>defence mechanisms<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">.<\/span><\/span><\/span><\/p>\n<p align=\"JUSTIFY\"><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>Polyphenols<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> include around <\/span><\/span><\/span><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7914517\/\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">8000 very different compounds<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, but the most widespread is the <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>flavonoids<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, which include anthocyanins, flavones, isoflavones and catechins, to name the best known. Then, there is the class of <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>phenolic acids and tannins<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, known as the <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>beneficial components<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> of red wine and tea, coffee, and cocoa. Polyphenols are mainly found in fruit skins and seeds, such as the berries and skins of grapes, or highly coloured fruits, such as berries and dried fruits and spices, such as cinnamon. And in the bark and generally in the woody parts of the <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>chestnut<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">.<\/span><\/span><\/span><\/p>\n<p align=\"JUSTIFY\"><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">Although <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>meat<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> has never been considered one of the best sources of polyphenols, <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>polyphenol supplementation in animal nutrition<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> is becoming widespread as a new strategy to <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>improve meat quality<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>prevent oxidative degradation<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> of lipids and proteins. Modulating animal rations to increase the nutritional value of their products is a technique that is becoming more and more established and offers numerous advantages. Just think of the <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>increase in omega-3 unsaturated fatty acids in meat<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and milk, <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>thanks to the addition of linseed or other supplements to the feed<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, which not only has <\/span><\/span><\/span><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0377840115300250?via%3Dihub\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">a positive effect on the animal&#8217;s immune system<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and well-being but also allows us to obtain better and <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>healthy meat<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, with less cholesterol and less saturated fat.<\/span><\/span><\/span><\/p>\n<p align=\"JUSTIFY\"><span class='bctt-click-to-tweet'><span class='bctt-ctt-text'><a href='https:\/\/x.com\/intent\/tweet?text=Supplementation%20with%20%23polyphenols%20in%20%23feeds%20is%20a%20new%20strategy%20to%20improve%20%23meat%20quality%20and%20prevent%20oxidative%20deterioration%20of%20%23lipids%20and%20%23proteins.&#038;related=SostenCarni&#038;url=https%3A%2F%2Fwww.carnisostenibili.it%2Fen%2F%3Fp%3D23592&#038;via=SostenCarni' target='_blank'rel=\"noopener noreferrer\">Supplementation with #polyphenols in #feeds is a new strategy to improve #meat quality and prevent oxidative deterioration of #lipids and #proteins. <\/a><\/span><a href='https:\/\/x.com\/intent\/tweet?text=Supplementation%20with%20%23polyphenols%20in%20%23feeds%20is%20a%20new%20strategy%20to%20improve%20%23meat%20quality%20and%20prevent%20oxidative%20deterioration%20of%20%23lipids%20and%20%23proteins.&#038;related=SostenCarni&#038;url=https%3A%2F%2Fwww.carnisostenibili.it%2Fen%2F%3Fp%3D23592&#038;via=SostenCarni' target='_blank' class='bctt-ctt-btn'rel=\"noopener noreferrer\">Share on X<\/a><\/span><\/p>\n<p align=\"JUSTIFY\"><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>Tannins<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, a special class of <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>polyphenols<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, are emerging as key players in transforming and enriching meat with beneficial properties. Although polyphenols are generally known for their <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>antioxidant properties<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, tannins are gaining recognition for their role in <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>animal nutrition<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> as part of a new strategy to <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>improve animal health<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>meat quality<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and protect lipids and proteins from oxidative damage. In particular, the <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>addition of tannins to ruminant diets<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> not only improves digestion but also helps to <\/span><\/span><\/span><a href=\"https:\/\/www.carnisostenibili.it\/en\/strong-reductions-in-methane-from-animal-feed\/\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>reduce methane emissions<\/b><\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, making this practice not only healthy but also sustainable.<\/span><\/span><\/span><\/p>\n<p align=\"JUSTIFY\"><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">Even <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>adding polyphenols<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> from various sources, such as oil mill effluent, <\/span><\/span><\/span><a href=\"https:\/\/bmcvetres.biomedcentral.com\/articles\/10.1186\/s12917-018-1513-1\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">has positively affected<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> meat&#8217;s quality characteristics and shelf life, <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>improving the fatty acid profile with a higher proportion of monounsaturated fats<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and reducing saturated fats and product oxidation. As wastewater from oil mills is a waste to be disposed of, <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>its reuse in animal feed is an innovative strategy for reusing waste from the agri-food industry<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">. Various agro-industrial by-products are widely used for this purpose, such as waste from cereal processing and the production of juices, wine and beer, i.e. pomace, peels, seeds and stalks, thus contributing to <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>the circularity of animal husbandry<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">.<\/span><\/span><\/span><\/p>\n<p align=\"JUSTIFY\"><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">Among the <\/span><\/span><\/span><a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jpn.12579\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">beneficial effects of polyphenols<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> in our organism, the antioxidant, <\/span><\/span><\/span><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S2214799316300133?via%3Dihub\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">anti-inflammatory<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>anti-tumour, anti-diabetic, anti-ageing<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, immunomodulatory, cardiovascular protection and prevention of <\/span><\/span><\/span><a href=\"https:\/\/link.springer.com\/article\/10.1007\/s12013-015-0558-z\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">neurodegenerative diseases<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> deserve attention. For this reason, <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>there is a growing interest in these new breeding techniques<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, which have potential benefits for <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>human and animal health<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">. Due to their antioxidant activity, <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>directly incorporating these natural by-products into animal feed rations improves the meat&#8217;s oxidative stability<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, shelf life and quality, as mentioned above.<\/span><\/span><\/span><\/p>\n<p align=\"JUSTIFY\"><span class='bctt-click-to-tweet'><span class='bctt-ctt-text'><a href='https:\/\/x.com\/intent\/tweet?text=The%20addition%20of%20%23tannins%20in%20the%20%23diet%20of%20%23ruminants%20not%20only%20positively%20affects%20their%20%23digestion%2C%20but%20also%20helps%20reduce%20%23methane%20%23emissions.&#038;related=SostenCarni&#038;url=https%3A%2F%2Fwww.carnisostenibili.it%2Fen%2F%3Fp%3D23592&#038;via=SostenCarni' target='_blank'rel=\"noopener noreferrer\">The addition of #tannins in the #diet of #ruminants not only positively affects their #digestion, but also helps reduce #methane #emissions. <\/a><\/span><a href='https:\/\/x.com\/intent\/tweet?text=The%20addition%20of%20%23tannins%20in%20the%20%23diet%20of%20%23ruminants%20not%20only%20positively%20affects%20their%20%23digestion%2C%20but%20also%20helps%20reduce%20%23methane%20%23emissions.&#038;related=SostenCarni&#038;url=https%3A%2F%2Fwww.carnisostenibili.it%2Fen%2F%3Fp%3D23592&#038;via=SostenCarni' target='_blank' class='bctt-ctt-btn'rel=\"noopener noreferrer\">Share on X<\/a><\/span><\/p>\n<p align=\"JUSTIFY\"><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">For example, <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>this antioxidant <\/b><\/span><\/span><\/span><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/18641184\/\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>has been found<\/b><\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b> in good concentrations in the meat of pigs fed with quercetin supplements<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, particularly in the offal. In <\/span><\/span><\/span><a href=\"https:\/\/citeseerx.ist.psu.edu\/document?repid=rep1&amp;type=pdf&amp;doi=060089e8d6845f987cda932d55c54273bd1dd3f5\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">chickens<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and <\/span><\/span><\/span><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/22818467\/\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">cattle<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">, <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>these techniques also enrich the meat and milk with substances that promote good health and strengthen the animals&#8217; immune defences<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and production efficiency. Incorporating <\/span><\/span><\/span><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27091079\/\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">natural antioxidants<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> such as polyphenols into the <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>diets of ruminants<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> and <\/span><\/span><\/span><a href=\"https:\/\/sciendo.com\/article\/10.1515\/aoas-2016-0042\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">monogastric<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> is now considered an <\/span><\/span><\/span><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/27138001\/\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\">effective strategy<\/span><\/span><\/a><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> for <\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"><b>improving the nutritional and already beneficial composition of meat<\/b><\/span><\/span><\/span><span style=\"color: #000000;\"><span style=\"font-family: Arial, serif;\"><span lang=\"en-GB\"> in response to the ever-increasing health demands of consumers. <\/span><\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Polyphenols are natural antioxidants with beneficial effects on human health and have recently become highly sought after by consumers in food products.<\/p>\n","protected":false},"author":2,"featured_media":23593,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[675],"tags":[5977,5976,5980,5981,5982,5983,5978,5979],"class_list":["post-23592","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-animal-welfare-en","tag-beneficial-meat-properties","tag-meat-polyphenols","tag-meat-tannins","tag-natural-antioxidants-in-meat","tag-polyphenols","tag-polyphenols-cattle-feed","tag-quercetin-animal-supplement","tag-tannins"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 5.0.2.1 - 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